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Protein Engineering by Yeast Surface Display
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Protein consensus-based surface engineering (ProCoS): a computer-assisted method for directed protein evolution.

Amol V Shivange1,2, Hans Wolfgang Hoeffken3, Stefan Haefner3

  • 1Lehrstuhl für Biotechnologie, RWTH Aachen University, Aachen, Germany.

Biotechniques
|December 13, 2016
PubMed
Summary

Protein consensus-based surface engineering (ProCoS) enhances enzyme performance by targeting variable surface regions. This method improved phytase pH stability significantly, demonstrating its utility for directed protein evolution.

Keywords:
ProCoSdirected evolutionmutagenesispH stabilityphytaseprotein engineering methodprotein surface engineering

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Area of Science:

  • Protein engineering
  • Enzyme evolution
  • Biotechnology

Background:

  • Protein consensus-based surface engineering (ProCoS) leverages conserved residues hypothesis for protein evolution.
  • Surface engineering targets highly variable regions for performance maximization.
  • ProCoS combines computational analysis and molecular biology for directed protein evolution.

Purpose of the Study:

  • To demonstrate the utility of ProCoS for directed evolution.
  • To engineer the surface of Yersinia mollaretii phytase (Ymphytase) for improved properties.

Main Methods:

  • ProCoS involves identifying conserved/variable regions, designing sequences, DNA recombination, and screening variants.
  • Multiple sequence alignment and structural models guide surface residue selection for mutagenesis.
  • Engineering-guided mutant libraries were screened.

Main Results:

  • Screening 1050 clones yielded a Ymphytase with 34 amino acid substitutions.
  • The engineered Ymphytase showed 3.8-fold higher pH stability at pH 2.8.
  • The enzyme retained 40% of wild-type specific activity and had increased surface negative charges.

Conclusions:

  • ProCoS is an effective method for protein surface engineering and directed evolution.
  • Surface engineering can significantly enhance enzyme stability under harsh conditions.
  • Increased surface negative charge may contribute to improved pH stability in engineered enzymes.