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Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Published on: May 1, 2019
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The CCAAT-binding complex (CBC) in Aspergillus species.
Peter Hortschansky1, Hubertus Haas2, Eva M Huber3
1Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Beutenbergstr. 11a, D-07745, Jena, Germany.
Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms
|December 13, 2016
Summary
The CCAAT binding complex (CBC) regulates fungal genes, sensing oxidative stress and iron levels. This vital transcription factor impacts fungal virulence, antifungal resistance, and biotechnological applications.
Area of Science:
- Molecular Biology
- Mycology
- Genetics
Background:
- The CCAAT binding complex (CBC) is a conserved eukaryotic transcription factor.
- CBC comprises a heterotrimeric core structure.
- It functions as a general transcriptional regulator in eukaryotes.
Purpose of the Study:
- To review the history and current knowledge of CBC gene regulatory functions, protein motifs, and structure in fungi.
- To focus on the role of CBC in Aspergillus species.
- To elucidate the diverse pathways regulated by CBC.
Main Methods:
- Literature review of scientific history and current knowledge.
- Focus on fungal species, particularly Aspergillus.
- Analysis of gene regulatory functions, protein motifs, and structure.
Main Results:
- CBC regulates diverse pathways beyond respiration, with a unifying rationale now emerging.
- CBC senses reactive oxygen species via oxidative cysteine modifications for redox regulation.
- CBC interacts with HapX to mediate adaptation to iron starvation and excess.
Conclusions:
- CBC is crucial for fungal virulence in hosts and antifungal resistance.
- CBC controls pathways in primary and secondary metabolism.
- CBC-mediated regulation is significant for biotechnology, agriculture, and infection medicine.
Keywords:
AspergillusCCAAT-binding complexHap complexHapXazole resistancegene regulationironvirulenceMore Related Videos
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