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Related Concept Videos

Heterochromatin02:38

Heterochromatin

18.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
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Heterochromatin02:38

Heterochromatin

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Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Histone Modification02:32

Histone Modification

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Euchromatin01:01

Euchromatin

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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
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Euchromatin01:01

Euchromatin

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Related Experiment Video

Updated: Mar 10, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

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H3K27 methylation: a promiscuous repressive chromatin mark.

Elizabeth T Wiles1, Eric U Selker1

  • 1Institute of Molecular Biology University of Oregon, Eugene, OR 97403-1229, USA.

Current Opinion in Genetics & Development
|December 13, 2016
PubMed
Summary

Polycomb Repressive Complex 2 (PRC2) targets genomic regions for histone H3 lysine 27 methylation (H3K27me). PRC2

Area of Science:

  • Epigenetics and Gene Regulation
  • Chromatin Biology
  • Molecular Biology

Background:

  • Polycomb Repressive Complex 2 (PRC2) catalyzes histone H3 lysine 27 methylation (H3K27me), a mark of facultative heterochromatin.
  • H3K27me is crucial for maintaining transcriptional repression during development.
  • The precise targeting mechanisms of PRC2 remain incompletely understood.

Purpose of the Study:

  • To elucidate the factors governing Polycomb Repressive Complex 2 (PRC2) targeting for H3K27me.
  • To investigate the role of chromatin context in directing PRC2 activity.
  • To understand the basis for cell-type-specific H3K27me patterns.

Main Methods:

  • Analysis of existing literature and emerging data on PRC2 function.
  • Comparative studies of H3K27me patterns across different cell types.

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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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Related Experiment Videos

Last Updated: Mar 10, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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  • Investigation of chromatin perturbations and their effects on H3K27me distribution.

    • Main Results:

    • DNA sequence alone is insufficient to determine H3K27me distribution.
    • H3K27me patterns vary significantly between different cell types.
    • Chromatin context, including histone modifications and nuclear organization, influences PRC2 targeting.

    Conclusions:

    • PRC2 targeting is not solely DNA-sequence dependent.
    • The chromatin environment plays a critical role in guiding PRC2 to specific genomic loci.
    • Understanding these interactions is key to deciphering epigenetic regulation and development.