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MOPS and coxsackievirus B3 stability

Steven D Carson1, Susan Hafenstein2, Hyunwook Lee2

  • 1Department of Pathology and Microbiology University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA.

Virology
|December 13, 2016
PubMed

Insights

MOPS (3-morpholinopropane-1-sulfonic acid) enhances coxsackievirus B3 stability in a concentration-dependent manner. This buffering agent interacts with viral capsid protein VP1, potentially stabilizing the virus by altering its structure.

Area of Science:

  • Virology
  • Biochemistry
  • Physical Chemistry

Background:

  • Coxsackievirus B3 (CVB3) is a significant human pathogen.
  • Maintaining virus stability in experimental conditions is crucial for research.
  • The buffering capacity of experimental media can influence virus stability.

Purpose of the Study:

  • To investigate the effect of MOPS (3-morpholinopropane-1-sulfonic acid) on CVB3 stability.
  • To elucidate the mechanism by which MOPS influences CVB3 stability.
  • To model the impact of MOPS and pH on virus decay kinetics.

Main Methods:

  • Experimental assessment of CVB3 stability in the presence of varying MOPS concentrations and pH.
  • Computer-simulated molecular docking to predict MOPS-CVB3 interactions.
  • Kinetic modeling to analyze virus decay rates.

Main Results:

  • MOPS significantly increased CVB3 stability, with effects being concentration-dependent.
  • Optimal virus stability was observed within a specific pH range (7.0-7.5) influenced by MOPS concentration.
  • Molecular docking revealed MOPS binding to a hydrophobic pocket in capsid protein VP1, forming ionic and hydrogen bonds.
  • Kinetic modeling confirmed the influence of MOPS and hydrogen ion concentrations on virus decay.

Conclusions:

  • MOPS directly associates with CVB3, enhancing its stability.
  • The stabilization mechanism likely involves MOPS binding to VP1 and altering capsid conformational dynamics.
  • MOPS is a valuable buffering agent for preserving CVB3 stability in research settings.
Keywords:
CoxsackievirusMOPSStabilitypH

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