Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Drug Product Stability

Drug Product Stability

The long-term stability of drug products is critical to ensuring their quality, safety, and effectiveness over time. Stability directly influences a product's ability to maintain its intended characteristics, ensuring it performs as expected during its intended shelf life. Key attributes such as drug potency, impurities, dissolution, and other physicochemical measures of performance are tested to assess stability. These parameters indicate how well the product retains its quality over time and...

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

Generic intravenous (IV) drugs are considered bioequivalent to their branded counterparts due to their 100% bioavailability upon administration. However, variations in stability among different drug products can significantly influence their therapeutic performance, even if they are pharmaceutically equivalent.Cefuroxime, a prophylactic antimicrobial, is often used as a single-dose IV injection for patients undergoing coronary artery bypass grafting surgery. A 3 g dose typically provides...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chronic Pain Severity Modifies Gait Biomechanics and Muscle Activation in Individuals with Chronic Ankle Instability.

Medicine and science in sports and exercise·2026

Same author

A Preliminary Study on the Efficacy and Safety of a Novel Digital Therapeutic Device: A Randomized Clinical Trial in South Korean Pediatric Patients With Attention-Deficit/Hyperactivity Disorder.

Psychiatry investigation·2026

Same author

Transferrin receptor 1 binds human parvovirus B19 VP1u to facilitate entry.

Nature communications·2026

Same author

Effects of Swelling on Static and Dynamic Postural Control and Patient-Reported Outcomes in Individuals With Chronic Ankle Instability.

Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine·2026

Same author

Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity.

Nature communications·2026

Same author

Distinct evolutionary patterns of endemic and emerging parvoviruses and the origin of a new pandemic virus.

Proceedings of the National Academy of Sciences of the United States of America·2026

Search research articles

Related Experiment Video

Updated: Mar 10, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

MOPS and coxsackievirus B3 stability.

Steven D Carson¹, Susan Hafenstein², Hyunwook Lee²

¹Department of Pathology and Microbiology University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA.

|December 13, 2016

Summary

This summary is machine-generated.

MOPS (3-morpholinopropane-1-sulfonic acid) enhances coxsackievirus B3 stability in a concentration-dependent manner. This buffering agent interacts with viral capsid protein VP1, potentially stabilizing the virus by altering its structure.

Keywords:

Coxsackievirus MOPS Stability pH

More Related Videos

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Published on: July 15, 2019

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Published on: February 20, 2021

Related Experiment Videos

Last Updated: Mar 10, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Published on: July 15, 2019

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Published on: February 20, 2021

Area of Science:

Virology
Biochemistry
Physical Chemistry

Background:

Coxsackievirus B3 (CVB3) is a significant human pathogen.
Maintaining virus stability in experimental conditions is crucial for research.
The buffering capacity of experimental media can influence virus stability.

Purpose of the Study:

To investigate the effect of MOPS (3-morpholinopropane-1-sulfonic acid) on CVB3 stability.
To elucidate the mechanism by which MOPS influences CVB3 stability.
To model the impact of MOPS and pH on virus decay kinetics.

Main Methods:

Experimental assessment of CVB3 stability in the presence of varying MOPS concentrations and pH.
Computer-simulated molecular docking to predict MOPS-CVB3 interactions.
Kinetic modeling to analyze virus decay rates.

Main Results:

MOPS significantly increased CVB3 stability, with effects being concentration-dependent.
Optimal virus stability was observed within a specific pH range (7.0-7.5) influenced by MOPS concentration.
Molecular docking revealed MOPS binding to a hydrophobic pocket in capsid protein VP1, forming ionic and hydrogen bonds.
Kinetic modeling confirmed the influence of MOPS and hydrogen ion concentrations on virus decay.

Conclusions:

MOPS directly associates with CVB3, enhancing its stability.
The stabilization mechanism likely involves MOPS binding to VP1 and altering capsid conformational dynamics.
MOPS is a valuable buffering agent for preserving CVB3 stability in research settings.