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Related Experiment Videos

Rat renal epinephrine synthesis.

M G Ziegler1, B Kennedy, H Elayan

  • 1Department of Medicine, University of California, San Diego Medical Center 92103.

The Journal of Clinical Investigation
|October 1, 1989
PubMed
Summary
This summary is machine-generated.

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The kidney synthesizes epinephrine (E), independent of the adrenal medulla. A unique renal enzyme, distinct from phenylethanolamine-N-methyltransferase (PNMT), produces E and is upregulated by treatments that reduce catecholamine levels.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Physiology

Background:

  • Plasma epinephrine (E) levels significantly decrease after adrenal demedullation.
  • Renal E levels, however, remain unaffected by adrenal demedullation and denervation, suggesting local synthesis.

Purpose of the Study:

  • To investigate the source and regulation of renal epinephrine synthesis.
  • To characterize the enzyme responsible for renal E production and its distinction from adrenal PNMT.

Main Methods:

  • Adrenal demedullation, 6-hydroxydopamine, and reserpine treatments in rats.
  • Infusion of [3H]methionine intravenously and into the renal artery.
  • Analysis of urinary and renal E and epinine levels.

Main Results:

Related Experiment Videos

  • Adrenal demedullation did not reduce renal E; treatments failed to lower renal E.
  • A distinct renal E-synthesizing enzyme, weakly inhibited by SKF 29661, was identified.
  • Renal E synthesis was induced by adrenal demedullation and further increased by combined treatments, while denervation normalized it.

Conclusions:

  • The kidney synthesizes significant amounts of renal and urinary epinephrine via an enzyme separate from adrenal PNMT.
  • This renal enzyme is induced by treatments that lower epinephrine and norepinephrine levels.