Neisseria in early stage of dental plaque

Neisseria in the early stage of dental plaque was studied. Two hundred seventeen strains of Neisseria were isolated from the 12-hour plaque of 9 subjects by using lactose-agar. The isolated strains were divided into 6 major groups with biological characteristics. One hundred twenty-one strains (56%) produced glycogen-like polysaccharide from sucrose. They were divided into 3 groups. Groups 1 and 2 were identified as N. mucosa and N. sicca, respectively. There were 106 strains of N. sica, being the most predominant of the species. The number of strains not producing polysaccharide was 96 (44%). Groups 4 and 5 were Branhamella and N. subflava. The absorption spectra of the ethanol extracts of the pigment in each group were similar and this result supported propriety of the classification by biological characteristics. The adhesiveness of Neisseria to the glass plate was examined. Approximately one-half of N. sica showed an adhesion capacity.