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Use of enzyme-developed immunoblots scanned by densitometry to compare lot-to-lot variability of Russian-thistle
L E Mansfield1, M Tan, G Martinez
1El Paso Institute for Medical Research and Development, University of Texas.
Abstract:
Two different lots of Russian-thistle extracts from five commercial sources were evaluated by isoelectric focusing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblots of Immobilon transfers from these separations. The immunoblots were developed by an enzyme-linked method and scanned with a densitometer. The two lots of a standardized extracts produced scans that suggested uniformity in allergenic components. Several of the nonstandarized extract lots also appeared quite comparable. With further refinement, immunoblotting with densitometric analysis appears to offer an attractive way to standardize allergen extracts.
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