Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dystrophic calcification and mineralization during bone induction: biochemical differences.

M E Nimni1

  • 1Department of Biochemistry, University of Southern California School of Medicine, Los Angeles.

Nihon Seikeigeka Gakkai Zasshi
|May 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The short-term therapeutic effect of recombinant human bone morphogenetic protein-2 on collagenase-induced lumbar facet joint osteoarthritis in rats.

Osteoarthritis and cartilage·2007
Same author

A modified rhTGF-beta1 and rhBMP-2 are effective in initiating a chondro-osseous differentiation pathway in bone marrow cells cultured in vitro.

Connective tissue research·2003
Same author

Cultures of ligament fibroblasts in fibrin matrix gel.

Connective tissue research·2003
Same author

Chondroitin sulfate and other sulfate containing chondroprotective agents may exhibit their effects by overcoming a deficiency of sulfur amino acids.

Osteoarthritis and cartilage·2003
Same author

Glutaraldehyde fixation revisited.

Journal of long-term effects of medical implants·2002
Same author

Production of a recombinant human basic fibroblast growth factor with a collagen binding domain.

Protoplasma·2001

Glutaraldehyde-crosslinked implants failed to calcify in older rats, unlike in younger ones. However, using embryonic cells with demineralized bone matrix (DBM) implants successfully induced bone formation in older rats.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Glutaraldehyde crosslinking of collagenous tissues affects their biological activity.
  • Bone formation capacity decreases with age, particularly in Fischer-344 rats.
  • Demineralized bone matrix (DBM) is an osteoinductive material.

Purpose of the Study:

  • To investigate the calcification of glutaraldehyde-crosslinked collagenous tissue implants in young and old rats.
  • To compare this to bone induction by non-crosslinked DBM.
  • To understand age-related reductions in bone formation and explore methods to overcome them.

Main Methods:

  • Subcutaneous implantation of glutaraldehyde-crosslinked DBM, tendon, and cartilage in young and old rats.
  • Analysis of implant calcification and Bone Gla Protein (BGP) levels.

Related Experiment Videos

  • Development of a DBM implant system with embryonic cells for implantation in aged rats.

    • Main Results:

    • Glutaraldehyde-crosslinked implants calcified in young rats but not in old rats, with minimal BGP accumulation.
    • Alkaline phosphatase activity was high in DBM but undetectable in crosslinked implants.
    • DBM implants containing embryonic calvaria or muscle cells successfully induced bone and/or cartilage formation in aged rats.

    Conclusions:

    • Glutaraldehyde crosslinking significantly impairs the osteoinductive potential of collagenous tissues in aged rats.
    • Age-related decline in bone formation can be overcome by using embryonic progenitor cells within a DBM scaffold.
    • This suggests a potential strategy for enhancing bone regeneration in elderly individuals.