Reversed-phase high-pressure liquid chromatography of some tryptamine derivatives

A quantitative reversed-phase high-pressure liquid chromatographic method is described for tryptamine, bufotenine, N-methyltryptamine and N,N-dimethyl tryptamine. All compounds were eluted with base-line separation within 15 min. The method is used to determine the tryptamine level in a leaf extract of Acacia podalyriaefolia A. Cunn. Satisfactory recoveries of tryptamine from plant material, and N,N-dimethyltryptamine from urine were obtained.