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Related Experiment Videos

Gene transfer experiments in cattle.

K Roschlau1, P Rommel, L Andreewa

  • 1Research Centre of Animal Production, Academy of Agricultural Sciences of GDR.

Journal of Reproduction and Fertility. Supplement
|January 1, 1989
PubMed
Summary

Gene transfer in cattle using four DNA constructs demonstrated varying efficiencies. Bovine papilloma virus DNA and alcohol dehydrogenase gene constructs showed higher success rates for genetic modification in bovine zygotes.

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Area of Science:

  • Animal Biotechnology
  • Molecular Biology
  • Reproductive Biology

Background:

  • Gene transfer techniques are crucial for genetic improvement in livestock.
  • Developing efficient methods for introducing foreign DNA into bovine zygotes is essential for agricultural applications.

Purpose of the Study:

  • To evaluate the efficiency of different gene constructs for gene transfer in cattle.
  • To determine the optimal timing for bovine zygote collection for gene injection.

Main Methods:

  • Four gene constructs were prepared: bovine papilloma virus DNA, Drosophila melanogaster alcohol dehydrogenase gene, and human/bovine growth hormone genes.
  • Bovine zygotes were collected 78-82 hours post-prostaglandin treatment and injected with foreign DNA.
  • Embryos were transferred to recipient heifers, and gene transfer efficiency was assessed using dot-blot hybridization.

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Main Results:

  • Gene transfer efficiencies were 3.2% for construct 1 (bovine papilloma virus DNA) and 6.9% for construct 2 (alcohol dehydrogenase gene).
  • Construct 4 (bovine growth hormone gene) resulted in a 0.8% gene transfer efficiency, with 14 calves born from 43 transferred embryos.
  • The optimal time for zygote collection was identified, and successful pregnancies were achieved.

Conclusions:

  • The study highlights varying efficiencies of different gene constructs for gene transfer in cattle.
  • The alcohol dehydrogenase gene construct showed the highest efficiency among those tested.
  • Optimized zygote collection and transfer protocols are vital for successful bovine gene transfer.