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Mutation induced by vibriophage PS166 infection changes biotype and phage type of Vibrio cholerae

S N Mitra1

  • 1Department of Bacterial Immunology, Indian Institute of Chemical Biology, Calcutta.

Journal of Medical Microbiology
|October 1, 1989
PubMed

Insights

Newly isolated vibriophage PS166 induced resistance in Vibrio cholerae MAK757. Mutants shifted biotype and gained new phage sensitivity while retaining their Ogawa serotype.

Area of Science:

  • Microbiology
  • Virology
  • Bacteriology

Background:

  • Vibrio cholerae causes cholera, a significant global health concern.
  • Bacteriophages (phages) are viruses that infect bacteria and can influence bacterial evolution.
  • Understanding phage-host interactions is crucial for controlling bacterial pathogens.

Purpose of the Study:

  • To investigate the effects of a novel vibriophage, PS166, on Vibrio cholerae (E1 Tor) strain MAK757.
  • To characterize the genetic and phenotypic changes in V. cholerae following resistance development to PS166.
  • To explore the implications of phage resistance on bacterial biotype and phage sensitivity.

Main Methods:

  • Isolation and characterization of a new vibriophage, PS166.
  • Experimental evolution of V. cholerae MAK757 to PS166 resistance.
  • Biotype determination and phage sensitivity profiling of resistant mutants.
  • Serotyping of parental and mutant strains.

Main Results:

  • Two PS166-resistant V. cholerae MAK757 mutants were successfully isolated.
  • These mutants transitioned from the E1 Tor biotype to the classical biotype.
  • Mutants acquired sensitivity to new temperate phages, distinct from the parental strain.
  • The Ogawa serotype of the V. cholerae mutants remained unchanged.

Conclusions:

  • Phage PS166 infection can drive significant evolutionary changes in Vibrio cholerae.
  • Resistance to one phage can lead to altered susceptibility to other phages.
  • The study provides insights into the molecular targets of phage PS166 and V. cholerae adaptation mechanisms.

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