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Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran
Arezoo Bozorgomid1, Naser Nazari2, Hamzeh Rahimi3
1Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Background:
We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.
Methods:
We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.
Results:
In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study.
Conclusion:
Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran.
