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RBM5 reduces small cell lung cancer growth, increases cisplatin sensitivity and regulates key

Julie J Loiselle1, Justin G Roy2, Leslie C Sutherland3

  • 1Biomolecular Sciences Program, Laurentian University, Sudbury, ON P3E 2C6, Canada.

Heliyon
|December 14, 2016
PubMed

Insights

Re-expressing the RBM5 tumor suppressor gene in small cell lung cancer (SCLC) cells slowed growth and increased chemotherapy sensitivity. This highlights RBM5

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • Small cell lung cancer (SCLC) is highly aggressive with poor prognosis.
  • The tumor suppressor gene RBM5 is downregulated in many cancers, but its role in SCLC is unclear.

Purpose of the Study:

  • To investigate the function of RBM5 in SCLC.
  • To determine the effects of RBM5 re-expression on SCLC cell lines and patient specimens.

Main Methods:

  • Utilized SCLC cell line (GLC20) with RBM5 deletion to create stable RBM5-expressing sublines.
  • Performed transcriptome and target identification studies.
  • Conducted RNA sequencing on paired SCLC patient tumor and non-tumor samples.
  • Performed functional studies on cell growth and chemosensitivity.

Main Results:

  • RBM5 re-expression regulated cell cycle and apoptosis in SCLC cells.
  • RBM5 significantly downregulated angiogenesis and cell adhesion pathways.
  • Decreased RBM5 expression was observed in SCLC patient tumors, correlating with pathway alterations seen in cell lines.
  • RBM5 expression slowed SCLC cell growth and enhanced cisplatin sensitivity.

Conclusions:

  • RBM5 plays a crucial role in maintaining the non-transformed state of lung cells.
  • RBM5 deletion contributes to SCLC development and progression.
  • RBM5 re-expression represents a potential therapeutic strategy for SCLC.
Keywords:
Cancer researchCell biology

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