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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.1K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Related Experiment Video

Updated: Mar 10, 2026

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
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Snapshot depth sensitive Raman spectroscopy in layered tissues.

Wei Liu, Yi Hong Ong, Xiao Jun Yu

    Optics Express
    |December 14, 2016
    PubMed
    Summary

    This study introduces a novel snapshot depth-sensitive Raman spectroscopy technique for analyzing layered tissues. The non-contact method rapidly captures spectra from multiple depths, showing potential for skin characterization and pharmaceutical quality monitoring.

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    Area of Science:

    • Optical Spectroscopy
    • Biomedical Optics
    • Materials Science

    Background:

    • Depth-sensitive Raman spectroscopy is crucial for analyzing layered biological tissues and materials.
    • Existing fiber-optic and lens-based Raman techniques face limitations in depth resolution, probe-sample contact, and acquisition speed.
    • Non-contact, rapid, and high-resolution depth profiling remains a significant challenge in Raman spectroscopy applications.

    Purpose of the Study:

    • To develop and validate a novel snapshot depth-sensitive Raman spectroscopy system.
    • To enable simultaneous, non-contact acquisition of Raman spectra from multiple depths within layered samples.
    • To assess the system's performance for skin characterization and pharmaceutical quality control.

    Main Methods:

    • A snapshot depth-sensitive Raman technique utilizing an axicon lens and a ring-to-line fiber assembly was developed.
    • A numerical ray-tracing tool was employed to optimize the system for signal collection efficiency and depth resolution.
    • The system was tested on skin phantoms, ex vivo pork tissues, and in vivo human thumbnails under controlled laser power.

    Main Results:

    • The developed system successfully acquired depth-sensitive Raman spectra from five distinct depths simultaneously and non-contact.
    • Optimization through numerical simulation balanced signal collection with depth resolution for skin measurements.
    • Effective depth profiling was demonstrated across various samples, including biological tissues and phantoms, within safety limits.

    Conclusions:

    • The snapshot depth-sensitive Raman spectroscopy technique offers a promising non-contact solution for analyzing layered tissues.
    • Its ability to rapidly acquire multi-depth spectra makes it suitable for clinical skin characterization and pharmaceutical applications.
    • The system overcomes limitations of previous methods, paving the way for advanced material and biological analysis.