Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amyloid Fibrils03:03

Amyloid Fibrils

12.4K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
12.4K
Amyloid Fibrils03:03

Amyloid Fibrils

6.9K
6.9K
Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.0K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zanubrutinib, Obinutuzumab, and Venetoclax in CLL: Long-Term Follow Up, MRD Kinetics, Retreatment, T-Cell Profiling, PKs.

Blood advances·2026
Same author

Deployment of AI-driven automated quality control of whole-slide images in a large tertiary cancer center.

Journal of pathology informatics·2026
Same author

Genomic features do not account for differences in multiple myeloma risk by ancestry.

Blood cancer discovery·2026
Same author

Genetic risk and immune dysregulation of classic Hodgkin lymphoma transformation of chronic lymphocytic leukemia/small lymphocytic lymphoma: a multicentric study.

medRxiv : the preprint server for health sciences·2026
Same author

The impact of metabolic dysfunction-associated steatotic liver disease on non-invasive fibrosis scores in patients with chronic hepatitis B: a multicenter retrospective study.

BMC gastroenterology·2026
Same author

Frequency and Distribution of Lymphatic Filariasis in Somalia: A Single-Center Experience.

Journal of tropical medicine·2026
Same journal

Basic Microglial Functions and How They Go Awry in Neurodegenerative Disease.

Annual review of pathology·2026
Same journal

Integration and Intersection of Cancer Metabolism with Epigenetic Pathways in Gliomas.

Annual review of pathology·2025
Same journal

The Role of Fibroblasts Across Inflammation and Immunity.

Annual review of pathology·2025
Same journal

The Role of MicroRNAs in Viral and Bacterial Infections.

Annual review of pathology·2025
Same journal

Role of Immune Cells in Hepatitis B Virus and Associated Sequelae.

Annual review of pathology·2025
Same journal

Pathology of the Coronary Arteries and Myocardium in Kawasaki Disease.

Annual review of pathology·2025
See all related articles

Related Experiment Video

Updated: Mar 10, 2026

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.8K

Amyloidosis: Insights from Proteomics.

Ahmet Dogan1

  • 1Departments of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065;

Annual Review of Pathology
|December 14, 2016
PubMed
Summary
This summary is machine-generated.

Amyloidosis, caused by abnormal protein deposits, affects organs like the heart and kidneys. Proteomics, a mass spectrometry technique, revolutionizes diagnosis and classification of this disease.

Keywords:
ALALECT2APOA1APOAC3ATTRB2MLECT2amyloidosismass spectrometryproteomics

More Related Videos

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

16.9K
Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
10:04

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

14.2K

Related Experiment Videos

Last Updated: Mar 10, 2026

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.8K
Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

16.9K
Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
10:04

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

14.2K

Area of Science:

  • Biochemistry
  • Pathology
  • Medical Diagnostics

Background:

  • Amyloidoses are a group of disorders characterized by abnormal protein folding and extracellular deposition.
  • These deposits cause tissue damage and organ dysfunction, affecting vital organs such as the heart, kidneys, and nerves.
  • Over 30 different proteins can cause amyloidosis, necessitating precise classification for effective treatment.

Purpose of the Study:

  • To highlight the transformative impact of mass spectrometry-based proteomics on amyloidosis diagnosis and classification.
  • To underscore the critical role of proteomic assays in clinical management and understanding amyloidosis pathogenesis.
  • To emphasize the discovery of new amyloid types, such as ALECT2 amyloidosis, through proteomic advancements.

Main Methods:

  • Application of mass spectrometry-based proteomic technologies for amyloid plaque analysis.
  • Utilizing proteomic assays for clinical management and biological information gathering in amyloidosis patients.
  • Investigating the pathogenesis of various amyloid types through proteomic insights.

Main Results:

  • Proteomic technologies have significantly advanced the diagnosis and classification of amyloidosis.
  • Proteomic assays provide crucial diagnostic and biological information for patient management.
  • Identification of novel amyloid types, including ALECT2 amyloidosis, has expanded the understanding of systemic amyloidosis prevalence.

Conclusions:

  • Proteomics has revolutionized the approach to amyloidosis, enabling precise diagnosis and classification.
  • Understanding the specific protein deposit is key to managing amyloidosis and requires advanced proteomic analysis.
  • ALECT2 amyloidosis is now recognized as a common form of systemic amyloidosis, particularly in North America, thanks to proteomic discoveries.