A novel pretreatment method combining sealing technique with direct injection technique applied for improving...

Area of Science:

• Clinical bioanalysis
• Biotechnology
• Pharmaceutical analysis

Background:

• Growing concerns regarding biosafety risks in clinical bioanalysis necessitate improved preparation methods.
• Existing methods may pose biosafety challenges, demanding innovative solutions.

Purpose of the Study:

• To develop a safe, simple, and effective pretreatment method for clinical bioanalysis.
• To enhance the biosafety profile of sample preparation techniques.

Main Methods:

• A novel pretreatment method was developed, integrating sealing and direct injection techniques.
• Antiviral drugs (adefovir and tenofovir) were used as model compounds.
• Calibration curves were prepared using standard solutions instead of spiked plasma.

Main Results:

• The method demonstrated high inter- and intraday precision (<4% RSD) and excellent extraction recoveries (99.4–100.7%).
• Utilizing standard solutions for calibration curves yielded more accurate results compared to plasma spiking.
• The developed method significantly improved biosafety in clinical bioanalysis.

Conclusions:

• The novel pretreatment method offers superior biosafety compared to traditional approaches.
• The method provides advantages such as enhanced precision, sensitivity, and recovery.
• This technique presents a feasible and practical platform for bioanalytical applications.