Establishment and partial characterization of a radiation-induced canine monocytic leukemic cell line (RK9ML-1)

A myeloid leukemic cell line, designated RK9ML-1, was established from a dog with acute radiation-induced monocytic leukemia. Based on cytochemical stains which reacted positively only with nonspecific esterase, morphological and ultrastructural characteristics which indicated the presence of phagocytic vacuoles and lysosomal bodies, and cell surface properties which indicated the presence of Fc receptors, all the findings support that RK9ML-1 is of monocytic lineage. Chromosomal analysis of the cell line indicated the cells to be hypodiploid with acrocentric autosomes characteristic of canine cells.