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Region-Specific Differences in Amyloid Precursor Protein Expression in the Mouse Hippocampus
Domenico Del Turco1, Mandy H Paul1, Jessica Schlaudraff1
1Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Germany.
Frontiers in Molecular Neuroscience
|December 15, 2016
Summary
Amyloid precursor protein (APP) expression differs regionally in the mouse hippocampus. Higher APP levels in area CA1 compared to the dentate gyrus may explain varied effects on synaptic plasticity.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Amyloid precursor protein (APP) is crucial for synaptic plasticity, learning, and memory.
- Previous studies show conflicting results regarding APP's role in hippocampal synaptic plasticity.
Purpose of the Study:
- Investigate regional differences in APP expression within the mouse hippocampus.
- Determine if these differences explain discrepancies in APP's role in synaptic plasticity.
Main Methods:
- Immunostaining, in situ hybridization (ISH), laser microdissection (LMD).
- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blotting.
Main Results:
- APP expression is approximately 1.7-fold higher in hippocampal Ammon's horn (area CA1) pyramidal cells than in dentate gyrus (DG) granule cells.
- This regional difference correlates with observed variations in APP's role in Hebbian plasticity.
Conclusions:
- Regional differences in APP expression are significant within the hippocampus.
- These expression variations likely account for the differing effects of APP deficiency on synaptic plasticity in area CA1 versus the DG.
