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Simultaneous extraction of beta-endorphin and leu- and met-enkephalins from human and rat plasma
S J Bhathena1, P M Smith, B W Kennedy
1CNL, USDA, Beltsville, Maryland.
Life Sciences
|January 1, 1989
Abstract:
A simple, rapid and reliable procedure is described to simultaneously concentrate and purify beta-endorphin, leu- and met-enkephalins from small volumes of human and rat plasma before radioimmunoassay is performed. It uses C18 Sep-Pak reverse phase cartridges. The effectiveness of different protease inhibitors in preventing degradation of opiates by plasma and different solvent systems for eluting opiates is also evaluated.