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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
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Multivalent pIX phage display selects for distinct and improved antibody properties
Lene S Høydahl1,2, Nicolay R Nilssen1,2, Kristin S Gunnarsen1
1Centre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital, N-0372 Oslo, Norway.
Scientific Reports
|December 15, 2016
Summary
This study introduces a novel phage display method using the pIX protein, improving antibody selection. This approach enhances the retrieval of specific antibodies with better properties by reducing bacterial toxicity during phage production.
Area of Science:
- Biotechnology
- Molecular Biology
- Immunology
Background:
- Phage display is a powerful technique for identifying antibody specificities.
- Optimizing lead antibody candidates from phage display libraries remains a significant challenge.
Purpose of the Study:
- To develop an improved phage display system for enhanced antibody lead candidate selection.
- To investigate the use of the pIX protein for antibody display, bypassing the SEC pathway.
Main Methods:
- Utilized the signal sequence-independent property of the pIX protein for antibody-capsid fusion and virion integration.
- Employed an engineered helper phage to enhance antibody pIX display and retrieval.
- Compared the pIX display system directly against the conventional pIII display system.
Main Results:
- The pIX display system demonstrated substantially improved retrieval of desired antibody specificities compared to pIII display.
- Antibodies selected via the pIX system exhibited favorable biophysical properties.
- Reduced E. coli host toxicity during phage propagation was observed due to the absence of a signal sequence in the pIX system.
Conclusions:
- The pIX combinatorial display platform offers a viable alternative for selecting high-affinity antibody binders.
- This method yields antibodies with improved stability and may have broad applicability in antibody engineering.
