Fabrication of Surface Protein-Imprinted Biofuel Cell for Sensitive Self-Powered Glycoprotein Detection

Glycoproteins are important biomarkers and therapeutic targets in clinical diagnostics. The conventional analytical methods for glycoprotein are usually faced with some challenges, such as the complex pretreatment of samples, poor availability, and limited stability of antibody, making them not suitable for point-of-care and on-site application. Herein, we demonstrate a novel miniaturized biofuel cells (BFCs)-based self-powered nanosensor for the specific and sensitive determination of glycoproteins in complex samples through the combination of boronate-affinity molecularly imprinted polymer (MIP) and the boronate affinity functionalized biliroxidase-carbon nanotube nanocomposites. The above MIP and the nanocomposites act as both signal probe and biocatalyst at the cathode. The as-obtained self-powered MIP-BFC-based biosensor can detect horseradish peroxidase (a type of glycoprotein) with a wide linear range of 1 ng/mL to 10 μg/mL and a very low detection limit of 1 ng/mL. Especially, it shows high tolerance for different interferences (e.g., sugars and other glycoproteins) and can even measure the α-fetoprotein level in serum samples. Moreover, it exhibits significant advantages over the conventional assays in terms of cost efficiency, stability, and speed, especially inexpensive instrument needed. Our novel approach for construction of the sensor paves a simple and economical way to fabricate portable devices for point-of-care and on-site application.