Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic Fe-Mo Dual Single-Atom Nanozymes: Enhanced Peroxidase-Like Activity and Colorimetric Detection of l-Penicillamine.

Inorganic chemistry·2026
Same author

Co-detection of human papillomavirus and human herpesvirus in women from Yunnan, China: associations with cervical lesion severity.

BMC infectious diseases·2026
Same author

The updated role of exosomes in cancer diagnosis and therapy.

Discover oncology·2026
Same author

Overcoming the blood-brain barrier: the role of functionalized carbon dots in treating central nervous system diseases.

International journal of pharmaceutics·2026
Same author

Characterization of multiple herpes viremia via next-generation sequencing in patients with lower respiratory tract infections: a retrospective cohort study.

BMC infectious diseases·2026
Same author

Recent Advances in Oral Drug Delivery Systems for BCS III Drugs.

Current issues in molecular biology·2026

Related Experiment Video

Updated: Mar 10, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.8K

DNA-Catalytically Active Gold Nanoparticle Conjugates-Based Colorimetric Multidimensional Sensor Array for Protein

Xiangcong Wei1, Zhengbo Chen1, Lulu Tan1

  • 1Department of Chemistry, Capital Normal University , Beijing, 100048, China.

Analytical Chemistry
|December 15, 2016
PubMed
Summary

This study introduces a novel protein sensing array using gold nanoparticles and DNA. The array accurately identifies proteins and their mixtures in samples, offering a simple, colorimetric detection method.

More Related Videos

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.2K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K

Related Experiment Videos

Last Updated: Mar 10, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.8K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.2K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K

Area of Science:

  • Nanotechnology
  • Biosensing
  • Analytical Chemistry

Background:

  • Gold nanoparticles (AuNPs) exhibit catalytic activity dependent on their surface area.
  • Protein interactions with AuNPs can mask their surface, altering catalytic performance.
  • Developing sensitive and selective protein detection methods is crucial for diagnostics.

Purpose of the Study:

  • To design and validate a protein sensing array utilizing functionalized gold nanoparticles.
  • To establish a colorimetric method for protein detection based on changes in AuNP catalytic activity.
  • To demonstrate the array's capability in distinguishing various proteins and their mixtures.

Main Methods:

  • Functionalizing gold nanoparticles (AuNPs) with single-strand oligonucleotides as receptors.
  • Utilizing the correlation between AuNP surface area masking by proteins and catalytic activity.
  • Employing a colorimetric assay (4-nitrophenol reduction) and color-change time (CCT) as the signal readout.
  • Using three DNA sequences (A15, C15, T15) as array elements and linear discriminant analysis (LDA) for data interpretation.

Main Results:

  • The sensing array successfully distinguished eleven proteins with 100% accuracy at the 30 nM level.
  • Discrimination of similar proteins (BSA, HSA), different proteins (BSA, Concanavalin), and protein mixtures with varying molar ratios was achieved with 100% accuracy.
  • The array demonstrated high accuracy (100%) in identifying proteins within human serum samples, validating its practical application.

Conclusions:

  • The developed gold nanoparticle-based sensing array offers a sensitive, selective, and visually interpretable method for protein detection.
  • The colorimetric response patterns and LDA provide a robust platform for complex biological sample analysis.
  • This approach holds significant potential for applications in diagnostics and biochemical analysis.