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Strain-Dependent Transcriptome Signatures for Robustness in Lactococcus lactis.

Annereinou R Dijkstra1,2,3, Wynand Alkema1,2,4, Marjo J C Starrenburg2

  • 1Kluyver Centre for Genomics of Industrial Fermentation, GA Delft, The Netherlands.

Plos One
|December 16, 2016
PubMed
Summary
This summary is machine-generated.

Fermentation conditions significantly impact Lactococcus lactis robustness against heat and oxidative stress. Different strains exhibit unique genetic responses, requiring tailored optimization for improved survival during processes like spray drying.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Food Science

Background:

  • Fermentation conditions critically influence the stress survival of Lactococcus lactis, a key bacterium in food production.
  • Previous studies identified gene groups linked to heat and oxidative stress robustness in L. lactis MG1363.
  • Diverse L. lactis strains (IL1403, KF147, SK11) exhibit varied robustness phenotypes, necessitating strain-specific investigations.

Purpose of the Study:

  • To determine if other L. lactis strains share similar or distinct transcriptome signatures for robustness compared to strain MG1363.
  • To apply a transcriptome-phenotype matching approach across multiple L. lactis strains under controlled fermentation conditions.
  • To identify genetic indicators of robustness for optimizing bacterial survival during industrial processes like spray drying.

Main Methods:

  • Subjected four L. lactis strains (MG1363, IL1403, KF147, SK11) to twelve fermentation conditions varying in salt, oxygen, temperature, and pH.
  • Harvested cells during the exponential growth phase for transcriptome analysis (gene expression profiling).
  • Assessed heat and oxidative stress survival phenotypes to correlate with gene expression data.

Main Results:

  • Fermentation conditions induced significant variations in heat and oxidative stress survival, up to five 10-log units, depending on the strain.
  • Highly strain-specific transcriptome signatures associated with robustness towards heat and oxidative stress were identified.
  • A small overlap in transcriptome responses revealed a generic signature including known stress genes (ctsR, lplL) and novel genes (nanE, transport proteins).

Conclusions:

  • Multiple genetic mechanisms contribute to bacterial robustness, underscoring the need for strain-specific optimization strategies.
  • Identified generic transcriptome markers (e.g., nanE, transport genes) can serve as indicators of robustness.
  • These findings can guide the selection of fermentation parameters to enhance L. lactis robustness for industrial applications like spray drying.