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Metabolomic screening using ESI-FT MS identifies potential radiation-responsive molecules in mouse urine
Daisuke Iizuka1,2, Susumu Yoshioka3, Hidehiko Kawai1,2
1Department of Experimental Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Journal of Radiation Research
|December 16, 2016
Summary
Researchers identified novel urinary biomarkers, including 1-methylhistamine, for early radiation exposure detection. This metabolomics approach using ESI-FT MS aids in rapid biodosimetry for radiation emergencies.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Toxicology
Background:
- High-throughput biodosimetric methods are crucial for radiation emergency medical response.
- Identifying reliable radiation-responsive molecules is essential for accurate dose assessment.
Purpose of the Study:
- To discover low-molecular-weight urinary molecules responsive to radiation using electrospray ionization Fourier transform mass spectrometry (ESI-FT MS).
- To develop a sensitive biodosimetry technique for early triage in radiation emergencies.
Main Methods:
- Comparative metabolomic analysis of mouse urine before and after irradiation.
- Utilizing electrospray ionization Fourier transform mass spectrometry (ESI-FT MS) for metabolite identification.
- Quantification of specific biomarkers using enzyme-linked immunosorbent assay (ELISA).
Main Results:
- Nine urinary metabolites were identified as radiation-responsive.
- Thymidine, thymine, and imidazoleacetic acid showed dose-dependent increases 8 hours post-irradiation.
- 1-methylhistamine demonstrated a significant, dose-dependent increase 72 hours post-irradiation, confirmed by ELISA.
Conclusions:
- Urinary metabolomics via ESI-FT MS is effective for identifying radiation-responsive molecules.
- Urinary 1-methylhistamine shows promise as a biomarker for acute, high-dose radiation exposure.
