Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A high-performance biodegradable Mg-Zn- Ca-Sn alloy with synergistic improvement of yield strength and corrosion rate achieved by precise control of extrusion and microstructural design.

Acta biomaterialia·2026
Same author

Interactive effects of forage source (alfalfa hay and wheat straw) and its delivery method (separate or mixed with starter diet) on growth, nutrient digestibility, rumen fermentation, and blood metabolites of Holstein dairy calves.

Tropical animal health and production·2026
Same author

Sex-specific retina-brain signatures link ERα/ERβ imbalance with gliosis in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same author

Prognostic biomarkers of distant metastasis following curative-intent resection of early-stage rectal cancer.

Clinical proteomics·2026
Same author

Patient induced pluripotent stem cells identify specificities of a reticular pseudodrusen phenotype in age-related macular degeneration.

Genome medicine·2026
Same author

Machine learning and neural network algorithms for prediction of C5 palsy after posterior surgery for ossification of posterior longitudinal ligament.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026

Related Experiment Video

Updated: Mar 10, 2026

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.5K

TMT One-Stop Shop: From Reliable Sample Preparation to Computational Analysis Platform.

Mehdi Mirzaei1, Dana Pascovici2, Jemma X Wu2

  • 1Faculty of Medicine and Health Sciences, Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, 2109, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|December 16, 2016
PubMed
Summary

This chapter details a quantitative proteomics workflow using tandem mass tags (TMT) for comprehensive data analysis. It covers sample preparation, labeling, and advanced multivariate analysis for robust biological insights.

Keywords:
Quantitative shotgun proteomicsSoftware workflowTMT

More Related Videos

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

4.7K
Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)
07:21

Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)

Published on: December 19, 2025

31.2K

Related Experiment Videos

Last Updated: Mar 10, 2026

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.5K
Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

4.7K
Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)
07:21

Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)

Published on: December 19, 2025

31.2K

Area of Science:

  • Proteomics
  • Biochemistry
  • Data Science

Background:

  • Quantitative proteomics enables large-scale protein expression analysis.
  • Tandem Mass Tags (TMT) are widely used for multiplexed quantitative proteomics.
  • Robust data analysis workflows are crucial for interpreting complex proteomics data.

Purpose of the Study:

  • To describe a comprehensive workflow for labeled quantitative proteomics using TMT.
  • To detail the entire process from sample preparation to multivariate data analysis.
  • To introduce the TMTPrepPro R package for enhanced data interpretation.

Main Methods:

  • Sample preparation and TMT labeling.
  • Peptide fractionation and mass spectrometry data acquisition.
  • Data processing using Proteome Discoverer and multivariate analysis with TMTPrepPro on GenePattern.

Main Results:

  • A step-by-step protocol for TMT-based quantitative proteomics.
  • Demonstration of data processing and interpretation for multi-run experiments.
  • Generation of various outputs for biological insights using TMTPrepPro.

Conclusions:

  • The described workflow provides a standardized approach for TMT quantitative proteomics.
  • The TMTPrepPro R package facilitates robust data analysis and interpretation.
  • This integrated workflow enhances the reliability and scope of proteomics studies.