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Long intragenic non-coding RNA lincRNA-p21 suppresses development of human prostate cancer

Xiaohai Wang1, Yuan Ruan1, Xingjie Wang1

  • 1Department of Urology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Cell Proliferation
|December 16, 2016
PubMed
Abstract

Insights

Long non-coding RNA p21 (lincRNA-p21) is down-regulated in prostate cancer, inhibiting tumor growth and promoting apoptosis. Its regulation of p53 downstream genes suggests a tumor-suppressive role in prostate cancer development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Prostate cancer is a leading malignancy in men globally, with complex underlying mechanisms.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in human cancer development.
  • The specific function of lincRNA-p21 in prostate cancer pathogenesis requires elucidation.

Purpose of the Study:

  • To investigate the role of lincRNA-p21 in the development of human prostate cancer.
  • To explore the molecular mechanisms by which lincRNA-p21 influences prostate cancer progression.

Main Methods:

  • Real-time PCR was used to assess lincRNA-p21 expression in prostate cancer cell lines and tissues.
  • Lentiviral vectors were employed to manipulate lincRNA-p21 levels, assessing effects on cell proliferation and apoptosis.
  • In vivo studies utilized a mouse xenograft model to evaluate lincRNA-p21's impact on tumor growth.
  • Western blotting and chromatin immunoprecipitation analyzed the relationship between lincRNA-p21 and p53 downstream genes.

Main Results:

  • LincRNA-p21 was found to be down-regulated in human prostate cancer tissues, correlating with advanced disease stage and poor survival.
  • Overexpression of lincRNA-p21 inhibited prostate cancer cell proliferation and colony formation in vitro and reduced tumor growth in vivo.
  • LincRNA-p21 was shown to promote apoptosis and induce p53 downstream gene expression by regulating p53 binding to their promoters.
  • Reduced expression of p53 downstream genes in malignant prostate tissues correlated with lower lincRNA-p21 levels.

Conclusions:

  • LincRNA-p21 acts as a tumor suppressor in human prostate cancer.
  • Its inhibitory effects are partly mediated through the regulation of p53 downstream gene expression.
  • Apoptotic activation by lincRNA-p21 also contributes to its anti-cancer role in prostate cancer development.
Keywords:
apoptosislincRNA-p21p53prostate cancer

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