Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

15.7K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
15.7K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.5K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

41.4K
Overview
41.4K
Homologous Recombination02:31

Homologous Recombination

64.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantum-machine-assisted drug discovery.

npj drug discovery·2026
Same author

Synthesis of the Phospha-Wittig Reagent Me<sub>3</sub>P = P(C<sub>2</sub>F<sub>5</sub>) and Generation of a Phospanyl-Wittig Derivative Me<sub>3</sub>P = C(CF<sub>3</sub>)P(C<sub>2</sub>F<sub>5</sub>)F.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Ki-67 shapes the nucleolus by anchoring chromatin via its amphiphilic properties.

The EMBO journal·2026
Same author

Stable Cyclic Peterson Olefination Intermediates.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Bis(trifluoromethyl)disulfide and the EtP<sub>4</sub> Phosphazene Base-Formation of a Bench-Stable [EtP<sub>4</sub>SCF<sub>3</sub>]<sup>+</sup>[SCF<sub>3</sub>]<sup>-</sup> Salt.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Bis(pentafluoroethyl)arsinous Acid and the Bis(pentafluoroethyl)arsinite─Synthesis, Structural Characterization and Transition-Metal Complexes.

Inorganic chemistry·2026

Related Experiment Video

Updated: Mar 10, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

14.7K

Profiling DNA damage response following mitotic perturbations.

Ronni S Pedersen1, Gopal Karemore1, Thorkell Gudjonsson1

  • 1Protein Signaling Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.

Nature Communications
|December 16, 2016
PubMed
Summary

Mitotic errors can cause DNA breakage in daughter cells. Replication stress may limit the proliferation of cells with abnormal karyotypes, impacting genome integrity.

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

4.2K
Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
09:39

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

1.0K

Related Experiment Videos

Last Updated: Mar 10, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

14.7K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

4.2K
Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
09:39

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

1.0K

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Genome integrity is crucial, maintained by coordinated DNA replication and chromosome segregation.
  • While replication stress is well-studied, the impact of mitotic errors on genome integrity is less understood.

Purpose of the Study:

  • To investigate the link between mitotic perturbations and DNA damage in daughter cells.
  • To identify genuine mitosis-born DNA damage events and their functional consequences.
  • To explore the role of replication stress in cells with abnormal karyotypes.

Main Methods:

  • Knockdown of 47 validated mitotic regulators.
  • Analysis of DNA breakage in daughter cells following mitotic errors.
  • Assessment of replication stress dynamics and proliferation rates.

Main Results:

  • A broad spectrum of mitotic errors correlates with increased DNA breakage.
  • Only a subset of these correlations are functionally linked.
  • DNA breakage after cytokinesis failure is preceded by accumulating replication stress.
  • Replication stress coincides with decreased cell proliferation.

Conclusions:

  • Mitotic aberrations lead to DNA breakage, with specific events identified.
  • Replication stress may act as a barrier to the propagation of cells with abnormal karyotypes.
  • This study provides a resource for assessing DNA damage dynamics associated with mitotic errors.