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Updated: Mar 10, 2026

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability.

Md Mehedi Hasan¹, Mohammad Wajih Alam¹, Khan A Wahid¹

¹Department of Electrical and Computer Engineering, University of Saskatchewan, Saskatoon, Canada.

|December 16, 2016

Summary

This summary is machine-generated.

A low-cost digital fluorescent microscope prototype was developed using commercial components to detect malignant tumor cells. This affordable system offers comparable performance to high-end microscopes for preclinical research.

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Area of Science:

Biomedical Engineering
Optical Microscopy
Cancer Research

Background:

Fluorescence microscopy is crucial for biological and medical research.
High-end commercial microscopes are often expensive and complex.
There is a need for cost-effective and portable microscopy solutions.

Purpose of the Study:

To develop a low-cost digital fluorescent microscope prototype.
To evaluate its efficacy in detecting malignant tumor cells.
To compare its performance with high-end commercial systems.

Main Methods:

Utilized commercial off-the-shelf (COTS) components for construction.
Integrated a torch with an excitation filter for fluorophore excitation.
Employed a dichroic mirror and barrier filter for signal isolation.
Connected the system to a computer via USB for real-time image capture and control.

Main Results:

Successfully detected malignant tumor cells using Alexa Fluor 488 conjugate dye.
The prototype demonstrated comparable features to high-end fluorescent microscopes.
Achieved significant reductions in cost, power consumption, weight, and size.

Conclusions:

The developed low-cost digital fluorescent microscope is a viable tool for preclinical cancer cell detection.
This prototype offers a cost-effective alternative for researchers and diagnostic applications.
Further development could enhance its capabilities for broader scientific use.