Engineering Fungal Nonribosomal Peptide Synthetase-like Enzymes by Heterologous Expression and Domain Swapping
Johannes W A van Dijk1, Chun-Jun Guo1, Clay C C Wang1,2
1Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , Los Angeles, California 90089, United States.
Abstract:
A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed the exchange of various domains in nonribosomal peptide synthase (NRPS)-like enzymes from Aspergillus terreus. The newly generated engineered enzymes are capable of producing compounds with different chemical structures than its parent enzyme in vivo. This work provides insight in the programing of nonribosomal peptide biosynthesis in filamentous fungi.
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