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Antibacterial Combination of Oleoresin from Copaifera multijuga Hayne and Biogenic Silver Nanoparticles Towards
Eliane S Otaguiri, Ana E B Morguette, Renata P Biasi-Garbin
1Departamento de Microbiologia, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445, km 380, CEP: 86057970, Londrina, Parana, Brazil.
Background:
Streptococcus agalactiae (group B Streptococcus - GBS) remains a leading cause of neonatal infections and an important cause of invasive infections in adults with underlying conditions.
Methods:
This study evaluated for the first time the effect of an oleoresin collected from Copaifera multijuga Hayne (copaiba oil) alone or in combination with silver nanoparticles produced by green synthesis using Fusarium oxysporum (AgNPbio) against planktonic and sessile cells of GBS isolated from colonized women.
Results:
Copaiba oil showed a dose-dependent bactericidal activity against planktonic GBS strains, including those resistant to erythromycin and/or clindamycin. Scanning and transmission electron microscopy of GBS treated with copaiba oil revealed morphological and ultrastructural alterations, displaying disruption of the cell wall and decreased electron density due to leakage of cytoplasmic content. Copaiba oil also exhibited antibacterial activity against biofilms of GBS strains, inhibiting their formation as well as the viability of mature biofilms. In addition, the combination of copaiba oil with AgNPbio resulted in a synergistic effect against planktonic cells and biofilm formation, reducing the minimal inhibitory concentration values of both compounds. No hemolytic activity was detected for both compounds.
Conclusion:
These results indicate the potential of copaiba oil, alone or in combination with AgNPbio, for the development of new alternative strategies for controlling GBS infections.
Insights
Copaiba oil and green-synthesized silver nanoparticles show promise against Streptococcus agalactiae (GBS). This combination effectively targets planktonic and biofilm GBS, offering new strategies for infection control.
Area of Science:
- Microbiology
- Nanotechnology
- Natural Products Chemistry
Background:
- Streptococcus agalactiae (group B Streptococcus - GBS) is a primary cause of neonatal infections.
- GBS also causes invasive infections in adults with comorbidities.
Purpose of the Study:
- To evaluate the antimicrobial potential of Copaifera multijuga Hayne (copaiba oil) and green-synthesized silver nanoparticles (AgNPbio) against GBS.
- To investigate the synergistic effects of copaiba oil and AgNPbio in combating GBS.
Main Methods:
- Assessing the activity of copaiba oil and AgNPbio against planktonic and sessile GBS.
- Utilizing scanning and transmission electron microscopy to observe GBS morphological changes.
- Evaluating the impact on GBS biofilm formation and mature biofilms.
- Determining synergistic effects and minimal inhibitory concentrations.
Main Results:
- Copaiba oil demonstrated dose-dependent bactericidal effects on planktonic GBS, including resistant strains.
- Microscopy revealed cell wall disruption and cytoplasmic leakage in GBS treated with copaiba oil.
- Copaiba oil inhibited GBS biofilm formation and reduced the viability of mature biofilms.
- The combination of copaiba oil and AgNPbio exhibited synergistic activity against planktonic GBS and biofilm formation.
- No hemolytic activity was observed for either compound.
Conclusions:
- Copaiba oil possesses significant antibacterial properties against GBS.
- The combination of copaiba oil and AgNPbio offers a synergistic approach to GBS control.
- These findings suggest potential for novel therapeutic strategies against GBS infections.
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