Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NMR Identification and Quantification of Galactinol in Green Coffea arabica: Implications for Traceability and Adulteration.

Journal of food science·2026
Same author

Determination of Histamine in Tuna Fish by a Rapid and Green DART-TQ MS Method.

Journal of mass spectrometry : JMS·2026
Same author

Metabolomic Profiling of Commercial Tomato Puree by One-Shot Mass Spectrometry-Based Analysis: A Qualitative Perspective.

Metabolites·2025
Same author

Doxorubicin-Cyclophosphamide Protocol in Dogs with Splenic Haemangiosarcoma and Haemoabdomen: A Retrospective Case Series.

Veterinary sciences·2025
Same author

Noncredible Complaints and Symptom Validity in Patients With Chronic Pain.

Pain research & management·2025
Same author

Whey Proteins and Bioactive Peptides: Advances in Production, Selection and Bioactivity Profiling.

Biomedicines·2025

Related Experiment Video

Updated: Mar 10, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.8K

Streamlining the analytical workflow for multiplex MS/MS allergen detection in processed foods.

Rosa Pilolli1, Elisabetta De Angelis1, Linda Monaci1

  • 1Institute of Sciences of Food Production, National Research Council of Italy (ISPA-CNR), Via Amendola 122/O, 70126 Bari, Italy.

Food Chemistry
|December 17, 2016
PubMed
Summary

A new sensitive method detects five common food allergens (egg, milk, soy, hazelnut, peanut) in processed foods like cookies. This optimized LC-MS/MS technique aids in protecting consumers with allergies.

Keywords:
11S globulin (PubChem CID: Q8W1C2)Alpha S1 casein (PubChem CID: P02662)Conarachin (PubChem CID: Q6PSU4)CookiesGlycinin (PubChem CID: P04776)Mass spectrometryMulti-allergen analysisOn-line SPEOvalbumin (PubChem CID: P01012)Processed foodSRM

More Related Videos

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.6K
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

17.0K

Related Experiment Videos

Last Updated: Mar 10, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.8K
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.6K
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

17.0K

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Food Safety

Background:

  • EU legislation mandates clear labeling of allergenic ingredients in pre-packaged foods.
  • Protecting allergic consumers necessitates sensitive analytical methods for detecting allergen traces.
  • Previous research established a foundation for developing advanced allergen detection techniques.

Purpose of the Study:

  • To develop and optimize a sensitive, label-free LC-MS/MS method for the multiplex detection of five key allergenic ingredients.
  • To validate the method in a complex food matrix (cookies) prepared at a laboratory scale.
  • To investigate the impact of baking on the detectability of specific allergen markers.

Main Methods:

  • Optimization of protein extraction and purification protocols for complex food matrices.
  • Development of a streamlined Selected Reaction Monitoring (SRM)-based LC-MS/MS assay.
  • Analysis of incurred cookies fortified with egg, skimmed milk, soy flour, hazelnut, and peanut.

Main Results:

  • An optimized, sensitive, label-free LC-MS/MS method was successfully developed for multiplex allergen detection.
  • The method demonstrated effectiveness in a complex processed food matrix (cookies).
  • The study investigated and reported on the effect of baking on allergen marker detection.

Conclusions:

  • The proposed LC-MS/MS method offers a sensitive and streamlined approach for detecting multiple food allergens.
  • This analytical tool can significantly contribute to ensuring food safety and protecting allergic individuals.
  • Further research can build upon these findings to enhance allergen monitoring in various food products.