Proteomic analysis of coffee grains exposed to different drying process
Kalynka Gabriella do Livramento1, Flávio Meira Borém1, Anderson Cleiton José1
1Federal University of Lavras, CEP 37200-000 Lavras, MG, Brazil.
Food Chemistry
|December 17, 2016
Summary
Drying coffee beans at 60°C alters their proteomic profile, reducing key proteins involved in sugar metabolism and stress response. This highlights how post-harvest processing impacts coffee quality and flavor markers.
Area of Science:
- Agricultural Science
- Biochemistry
- Food Science
Background:
- Post-harvest processes significantly influence coffee bean composition and beverage quality.
- Identifying molecular markers is crucial for characterizing coffee flavor.
- Understanding biochemical changes during drying is essential for quality control.
Purpose of the Study:
- To evaluate proteomic profile changes in *C. arabica* coffee beans.
- To compare the effects of yard drying versus mechanical drying at 60°C.
- To identify potential protein markers related to coffee quality.
Main Methods:
- Proteomic analysis of *C. arabica* coffee beans.
- Comparison of pulped and natural coffee beans.
- Drying methods: yard drying vs. mechanical drying at 60°C.
Main Results:
- Mechanical drying at 60°C altered the proteomic profile compared to yard-dried beans.
- A reduction in abundant proteins was observed in beans dried at 60°C.
- Proteins involved in sugar metabolism and stress response were notably affected.
Conclusions:
- Post-harvest drying methods significantly impact coffee bean proteomic profiles.
- Changes in protein abundance correlate with coffee quality.
- Proteomic analysis is a valuable tool for detecting biochemical alterations in coffee processing.
Keywords:
Acetonitrile (Pubmed CID: 6342)Ammonium bicarbonate (Pubmed CID: 14013)Ammonium sulfate (Pubmed CID: 6097028)Beverage qualityCoffea arabicaCoomassie blue G250 (Pubmed CID: 6333920)Dithiothreitol (Pubmed CID: 19001)Iodoacetamide (Pubmed CID: 3727)ProteomeThiourea (Pubmed CID: 2723790)Two-dimensional electrophoresisUrea (Pubmed CID: 1176)α-Cyano-4-hydroxycinnamic acid (Pubmed CID: 5328791)More Related Videos
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