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Updated: Mar 10, 2026
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
Published on: April 13, 2022
Data supporting regulating temporospatial dynamics of morphogen for structure formation of the lacrimal gland by
Ya-Chuan Hsiao1, Tsung-Lin Yang2
1Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan, ROC; Department of Ophthalmology, Zhongxing Branch, Taipei City Hospital, Taipei, Taiwan, ROC; Department of Ophthalmology, College of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
Abstract:
The lacrimal gland is responsible for tear synthesis and secretion, and is derived from the epithelia of ocular surface and generated by branching morphogenesis. The dataset presented in this article is to support the research results of the effect of chitosan biomaterials on facilitating the structure formation of the lacrimal gland by regulating temporospatial dynamics of morphogen. The embryonic lacrimal gland explants were used as the standard experimental model for investigating lacrimal gland branching morphogenesis. Chitosan biomaterials promoted lacrimal gland branching with a dose-dependent effect. It helped in vivo binding of hepatocyte growth factor (HGF) related molecules in the epithelial-mesenchymal boundary of emerging epithelial branches. When mitogen-activated protein kinase (MAPK) or protein kinase B (Akt/PKB) inhibitors applied, the chitosan effects reduced. Nonetheless, the ratios of MAPK and Akt/PKB phosphorylation were still greater in the chitosan group than the control. The data demonstrated here confirm the essential role of HGF-signaling in chitosan-promoted structure formation of the lacrimal gland.
