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A lateral flow assay (LFA) for the rapid detection of extraparenchymal neurocysticercosis using cerebrospinal fluid
Agnes Fleury1, Patricia Sastre2, Edda Sciutto3
1Unidad de Neuroinflamación (Instituto de Investigaciones Biomédicas-UNAM, Instituto Nacional de Neurología y Neurocirugia, Facultad de Médicina-UNAM), Insurgentes Sur 3877, Colonia La Fama, delegación Tlalpan, Mexico D.F, Mexico; Neurocysticercosis Clinic, Instituto Nacional de Neurología y Neurocirugia, Mexico D.F, Mexico.
Abstract:
A lateral flow assay (LFA) for the diagnosis and monitoring of extraparenchymal neurocysticercosis, has been developed. The assay is based on the use of the monoclonal antibody HP10, and when applied to cerebrospinal fluid, correctly identified 34 cases of active extraparenchymal neurocysticercosis, but was negative with 26 samples from treated and cured neurocysticercosis patients and with 20 samples from unrelated neurological diseases. There was complete agreement between the HP10 Ag-ELISA results and the HP10-LFA. The HP10-LFA thus has utility for diagnosis and treatment of extraparenchymal neurocysticercosis, frequently a more dangerous form of the infection.
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