Related Experiment Video

Updated: Aug 13, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 27, 2013

Isolation and characterization of two immunoregulatory factors--interleukin 2 inhibitors

Y Y Wu, J Zhang, Y H Zhang

    Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-Kuo I Hsueh K'O Hsueh Yuan, Chung-Kuo Hsieh Ho I K'O Ta Hsueh Hsueh Pao
    |January 1, 1989
    PubMed

    Supernatants of cultured gibbon T lymphoma cells MLA144 were shown to contain two suppressive factors (IF50 and IF80) isolated by ammonium sulfate precipitation, ion-exchange chromatography and gel filtration. Both factors had similar activities: inhibition of IL 2 production by human peripheral blood mononuclear cells, inhibition of IL 2 responsiveness of human lymphoblasts and mouse thymocytes, and inhibition of proliferation of Con A-stimulated mouse splenocytes. However, they did not affect E receptors. Fc receptors or phagocytosis. The physico-chemical properties of these two factors were also different. It is of special interest to note that the MLA144 cell line which spontaneously secretes IL 2 also generates IL 2 inhibitors. This offers an experimental model for investigation of the intracellular immunoregulatory mechanism of IL 2 expression.

    More Related Videos

    In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function09:26

    In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

    Published on: October 16, 2013

    Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD22608:30

    Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

    Published on: May 10, 2022

    Related Concept Videos

    T Cell Types and Functions01:24

    T Cell Types and Functions

    When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
    Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...