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Use of the APTIMA Combo 2 Assay and a Secondary Algorithm to Detect and Confirm Chlamydia trachomatis in Rectal-Only
Kanti Pabbaraju1, Sallene Wong, Kara Gill
1From the *Provincial Laboratory for Public Health, Calgary, Alberta; †National Microbiology Laboratory, Public Health Agency of Canada; ‡Department of Medical Microbiology, Faculty of Health Sciences, University of Manitoba; §STI Centralized Services, Alberta Health Services; ¶Department of Medicine, University of Alberta; ∥Department of Medicine, **Department of Microbiology, Immunology and Infectious Diseases, University of Calgary; ††Provincial Laboratory for Public Health, Edmonton; and ‡‡Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
Abstract:
We sought to confirm the results of 81 rectal specimens positive for Chlamydia trachomatis by the APTIMA Combo 2 assay among patients with concurrently collected negative genitourinary specimens. A total of 79 (97.5%) samples were confirmed by the APTIMA single target assay and/or sequencing of the C. trachomatis ompA gene.
