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Related Experiment Video

Updated: Mar 10, 2026

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

Published on: May 24, 2024

Making the cut: Innovative methods for optimizing perfusion-based migration assays.

Andrew W Holt¹, William E Howard², Elizabeth T Ables³

¹Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, North Carolina.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|December 17, 2016

Summary

This summary is machine-generated.

New 3D printing and laser capture microdissection methods enable precise study of cell migration under fluid shear stress, overcoming limitations of traditional techniques for better understanding flow-mediated disorders.

Keywords:

3D printing cell migration computational fluid dynamics fluid shear stress laser capture microdissection smooth muscle cells

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Last Updated: Mar 10, 2026

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

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A Cost Effective and Adaptable Scratch Migration Assay

A Cost Effective and Adaptable Scratch Migration Assay

Published on: June 30, 2020

Area of Science:

Cell biology
Biotechnology
Biomedical engineering

Background:

Fluid shear stress significantly impacts cell cytoskeletal structure and migration.
Studying cell migration under flow requires preserving cytoskeletal adaptations.
Current in vitro perfusion systems have limitations in studying cell migration post-perfusion.

Purpose of the Study:

To identify limitations in current cell migration assays.
To introduce novel methods using 3D printing and laser capture microdissection (LCM) for cell migration studies.
To enable accurate cell migration analysis in conjunction with perfusion experiments.

Main Methods:

Development of residue-free 3D printed inserts for controlled cell seeding and shear stress application.
Utilized laser capture microdissection (LCM) for precise, touchless wound creation in microchannels.
Compared outcomes with traditional cell migration assay methods.

Main Results:

3D printed inserts provide accurate seeding, increased yield, and relevant shear stress levels.
LCM creates custom wound areas comparable to traditional methods but without accessibility issues.
Both new methods yield similar results to traditional assays but avoid compromising cell phenotypes.

Conclusions:

Additive manufacturing (3D printing) and LCM are effective tools for studying cell migration under perfusion.
These methods overcome limitations of traditional assays, preserving cell cytoskeletal integrity.
The innovative tools are valuable for research into flow-mediated disorders and other perfusion-based cell migration models.