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Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

pH-switchable bacteria detection using zwitterionic fluorescent polymer.

Khoerunnisa¹, Zihnil A I Mazrad¹, Insik In²

¹Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea.

Biosensors & Bioelectronics

|December 17, 2016

Summary

This summary is machine-generated.

A novel zwitterionic fluorescent polymer detects pathogenic bacteria with high pH sensitivity. This polymer, BOD/BE-PSM, binds bacteria and changes fluorescence, enabling sensitive detection across various pH levels.

Keywords:

Gram-negative bacteria Gram-positive bacteria Ionic complex Zwitterionic fluorescent polymer

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Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

Published on: February 17, 2014

Area of Science:

Polymer Chemistry
Biotechnology
Analytical Chemistry

Background:

Pathogenic bacteria detection remains a critical challenge in healthcare.
Developing sensitive and specific detection methods is essential for early diagnosis and treatment.
Zwitterionic materials offer unique properties for biological applications.

Purpose of the Study:

To construct a zwitterionic fluorescent polymer for sensitive detection and imaging of pathogenic bacteria.
To investigate the pH-dependent fluorescence response of the polymer for bacterial identification.
To evaluate the polymer's stability and limit of detection for bacteria.

Main Methods:

Synthesis of a zwitterionic fluorescent polymer (BOD/BE-PSM) using boron dipyrromethane (BODIPY) and bromoethane conjugated with poly(sulfobetaine methacrylate).
Incubation of the polymer with bacteria to observe binding via ionic complex formation.
Analysis of fluorescence changes in response to varying pH levels (6.0, 7.4, 9.0) using fluorescence spectroscopy and confocal microscopy.

Main Results:

The zwitterionic fluorescent polymer (BOD/BE-PSM) effectively bound to both gram-positive and gram-negative bacteria within 1 hour.
A pH-dependent fluorescence on/off system was observed, linked to changes in the polymer's hydrophilicity/hydrophobicity.
The detection system demonstrated good stability with a limit of detection of 1 mg/mL, confirmed by fluorescence spectroscopy and imaging.

Conclusions:

The developed zwitterionic fluorescent polymer is a promising tool for sensitive bacteria detection and imaging.
The polymer's pH-responsive fluorescence allows for versatile application in bacterial analysis across a wide pH range.
This approach offers a novel strategy for developing advanced biosensors for pathogenic bacteria.