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Updated: Mar 10, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Published on: August 3, 2021
Phosphatidyl-hydroxytyrosol and phosphatidyl-tyrosol bilayer properties
Kervin O Evans1, David L Compton1
1Renewable Products Research Unit, USDA, Agriculture Research Service, National Center for Agricultural Utilization Research Center, 1815N. University Street, Peoria, IL 61604, USA.
Abstract:
Hydroxytyrosol and tyrosol phospholipids were enzymatically synthesized and investigated for their bilayer properties. Dynamic light scattering demonstrated that hand extrusion at 100nm consistently resulted in liposomes of nearly 85nm diameter for both phosphatidyl-hydroxytyrosol (DOPHT) and phosphatidyl-tyrosol (DOPT). Transmission electron microscopy showed DOPT and DOPHT liposomes extruded at 100-nm to be spherical and non-distinctive from one another. Zeta potential measurements resulted in surface charges<-25mV, demonstrating both DOPT and DOPHT form highly stable liposomes. Quartz crystal microbalance with dissipation monitoring measurements demonstrated that liposomal adsorption was dependent on a combination of DOPT (or DOPHT) mole-percent and calcium ions concentration. Fluorescence anisotropy measurements indicated that melting temperatures of DOPT and DOPHT were below 4°C, suggesting that adsorption behavior and liposome formation was limited by electrostatic interactions and not gel-state formation.
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