Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioremediation00:46

Bioremediation

22.7K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
22.7K
Biofilms01:29

Biofilms

1.7K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Few-mode fiber based Raman distributed temperature sensing.

Optics express·2017
Same author

Measuring Complexity and Predictability of Time Series with Flexible Multiscale Entropy for Sensor Networks.

Sensors (Basel, Switzerland)·2017
Same author

Tripodal S-Ligand Complexes of Copper(I) as Catalysts for Alkene Aziridination, Sulfide Sulfimidation, and C-H Amination.

Inorganic chemistry·2017
Same author

5-aminolevulinic acid-mediated photodynamic therapy and its strain-dependent combined effect with antibiotics on Staphylococcus aureus biofilm.

PloS one·2017
Same author

Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp).

Journal of analytical methods in chemistry·2017
Same author

Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.

International journal of oncology·2017

Related Experiment Video

Updated: Mar 10, 2026

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
13:16

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Published on: December 24, 2014

11.0K

Pyrene biodegradation with layer-by-layer assembly bio-microcapsules.

Fucai Deng1, Zhengfang Zhang2, Chen Yang2

  • 1College of Environment and Energy, South China University of Technology, Guangzhou 510006, China; School of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.

Ecotoxicology and Environmental Safety
|December 18, 2016
PubMed
Summary

Encapsulating bacteria in chitosan/alginate bio-microcapsules significantly enhances polycyclic aromatic hydrocarbon (PAH) biodegradation. This biotechnology offers improved bacterial tolerance to harsh environmental conditions for effective pollutant removal.

Keywords:
Bio-microcapsulesBiodegradationEnvironmental stressEnvironmental toleranceLayer-by-layer assemblyPyrene

More Related Videos

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
08:21

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Published on: May 16, 2022

6.0K
Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
13:38

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

Published on: May 10, 2013

31.6K

Related Experiment Videos

Last Updated: Mar 10, 2026

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
13:16

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Published on: December 24, 2014

11.0K
Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
08:21

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Published on: May 16, 2022

6.0K
Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
13:38

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

Published on: May 10, 2013

31.6K

Area of Science:

  • Environmental Biotechnology
  • Bioremediation
  • Materials Science

Background:

  • Biotechnology offers a promising approach for removing polycyclic aromatic hydrocarbons (PAHs) from the environment.
  • Free-living bacteria often exhibit sensitivity to environmental stressors, limiting their biodegradation efficiency for organic pollutants like PAHs.
  • Developing robust bacterial systems is crucial for overcoming these limitations and enhancing bioremediation capabilities.

Purpose of the Study:

  • To investigate the efficacy of chitosan/alginate bio-microcapsules in enhancing pyrene (PYR) biodegradation under challenging environmental conditions.
  • To assess the tolerance and performance of encapsulated bacteria compared to free-living bacteria in PAH removal.

Main Methods:

  • Chitosan/alginate bio-microcapsules were fabricated using the layer-by-layer (LBL) assembly technique.
  • Morphological and surface analyses (mesopore assessment) were conducted on the prepared bio-microcapsules.
  • Biodegradation experiments were performed using encapsulated bacteria and free bacteria to evaluate PYR removal rates under various stress conditions (high concentration, low pH, extreme temperatures, high salt).

Main Results:

  • LBL assembly successfully produced flake-shaped bio-microcapsules with significant mesoporous structures.
  • Encapsulated bacteria achieved 95% PYR removal within 3 days, substantially outperforming free bacteria (59%) at 10 mgL⁻¹.
  • Encapsulated bacteria demonstrated 1-6 times higher PYR biodegradation rates under harsh conditions (50 mgL⁻¹ PYR, pH 3, 10°C/40°C, high salt) compared to free cells.

Conclusions:

  • Bacteria encapsulated within chitosan/alginate bio-microcapsules exhibit significantly enhanced tolerance to environmental stressors.
  • The LBL-assembled bio-microcapsule system is a promising candidate for effective bioremediation of organic pollutants like PAHs.
  • This approach improves bacterial resilience, paving the way for more efficient environmental cleanup strategies.