Related Experiment Video
Updated: Mar 10, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
Published on: November 20, 2013
Optical devices for the detection of cyanide in water based on ethyl(hydroxyethyl)cellulose functionalized with
Leandro Guarezi Nandi1, Celso Rodrigo Nicoleti1, Vanderléia Gava Marini1
1Departamento de Química, Universidade Federal de Santa Catarina, UFSC, CP 476, Florianópolis, SC, 88040-900, Brazil.
Abstract:
Films of three polymers, based on ethyl(hydroxyethyl)cellulose functionalized with protonated perichromic dyes, were used for anion sensing. The polymer functionalized with protonated Brooker's merocyanine acts as a chromogenic/fluorogenic system for the selective detection of cyanide in water. An increase of >28 times was verified for the fluorescence lifetime of the sensing units in the polymer in comparison with protonated Brooker's merocyanine in water. Moreover, an increase in the pKa values was verified for the sensing units in the polymers. Data suggest that the hydrocarbonic polymeric chains provide an adequate microenvironment to protect the sensing unit from bulk water. The other polymer, functionalized with an iminophenol, also showed high selectivity for cyanide (detection limit=9.36×10-6molL-1 and quantification limit=3.12×10-5molL-1). The polymer functionalized with azophenol units is unable for the detection of cyanide, due to the low pKa value verified for its chromogenic units.
More Related Videos
10:57Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
Published on: February 7, 2017
09:51TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples
Published on: September 19, 2025
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Types of Detectors-II
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Gas Chromatography: Overview of Detectors
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...