Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

41.4K
Overview
41.4K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.5K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
Ribozymes02:47

Ribozymes

13.6K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a BM7G(TKO/hCD46/hCD55/hTHBD/hEPCR) donor pig with endogenous promoter-driven transgenes for xenotransplantation.

Frontiers in immunology·2026
Same author

Incidence of Dengue Fever in Pakistan.

PloS one·2026
Same author

[Synchronous detection test strip of multiple exogenous mycotoxins in Chinese medicinal materials based on flower-like gold nanoparticles].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2026
Same author

Single-atom vanadium anchored on N-defects g-C<sub>3</sub>N<sub>4</sub> as intrinsic peroxidase mimetics for colorimetric assay of sarcosine.

Mikrochimica acta·2026
Same author

Zhi-Zi-Chi Decoction Alleviates Depressive-like Behaviors by Regulating Gut Microbiota and Targeting the AMPK/PI3K-TOR Pathway via Its Metabolite Protocatechuic Acid.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Functional characterization and catalytic mechanism of a C/N-selective methyltransferase MaMT4 involved in 1-deoxynojirimycin-type alkaloids biosynthesis in mulberry leaves.

Plant physiology and biochemistry : PPB·2026

Related Experiment Video

Updated: Mar 10, 2026

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by GalliumIII and H3 5,10,15-trispentafluorophenylcorroles
09:00

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by GalliumIII and H3 5,10,15-trispentafluorophenylcorroles

Published on: March 18, 2015

12.1K

Rutin-Nickel Complex: Synthesis, Characterization, Antioxidant, DNA Binding, and DNA Cleavage Activities.

Aun Raza1, Shumaila Bano2, Xiuquan Xu1

  • 1School of pharmacy, Jiangsu University, Zhenjiang, 212013, China.

Biological Trace Element Research
|December 19, 2016
PubMed
Summary

The synthesized rutin-nickel (II) complex exhibits enhanced antioxidant activity and effectively cleaves DNA. This metal complex shows potential as a nuclease agent, demonstrating significant DNA degradation capabilities.

Keywords:
AntioxidantDNA bindingDNA cleavageRutin–nickel (II) complex

More Related Videos

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

12.8K
Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

8.2K

Related Experiment Videos

Last Updated: Mar 10, 2026

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by GalliumIII and H3 5,10,15-trispentafluorophenylcorroles
09:00

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by GalliumIII and H3 5,10,15-trispentafluorophenylcorroles

Published on: March 18, 2015

12.1K
The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

12.8K
Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

8.2K

Area of Science:

  • Inorganic Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • Rutins are flavonoids with known antioxidant properties.
  • Metal complexes can exhibit enhanced biological activities compared to their parent ligands.
  • DNA interaction studies are crucial for understanding potential therapeutic applications.

Purpose of the Study:

  • To synthesize and characterize a novel rutin-nickel (II) complex.
  • To evaluate the antioxidant capacity of the complex.
  • To investigate the interaction of the complex with DNA and its DNA cleavage potential.

Main Methods:

  • Synthesis and characterization using elemental analysis, spectroscopy (UV-Vis, IR, NMR), mass spectrometry, TG-DSC, SEM, and conductivity measurements.
  • Antioxidant activity assessed using the DPPH radical scavenging assay.
  • DNA interaction studied via fluorescence spectroscopy and agarose gel electrophoresis.

Main Results:

  • The rutin-nickel (II) complex was successfully synthesized with a 1:2 metal-ligand ratio and confirmed as a non-electrolyte.
  • The complex displayed superior radical scavenging activity compared to free rutin.
  • The complex demonstrated moderate DNA intercalation, competed with ethidium bromide, and induced dose-dependent cleavage of pBR 322 DNA via a hydrolytic pathway.

Conclusions:

  • The synthesized rutin-nickel (II) complex possesses enhanced antioxidant properties.
  • The complex exhibits significant DNA binding and cleavage activity, indicating potential as a DNA nuclease.
  • Further research into this complex could lead to novel therapeutic agents.