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Positive Expression of Human Cytomegalovirus Phosphoprotein 65 in Atherosclerosis

Zhe Wang1, Jun Cai1, Mingming Zhang1

  • 1Department of Cardiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.

Biomed Research International
|December 20, 2016
PubMed

Insights

Human cytomegalovirus (HCMV) infection, indicated by pp65 protein, is linked to atherosclerosis. While viral DNA wasn't found, pp65 presence suggests HCMV may play a role in artery disease development.

Area of Science:

  • Vascular Biology
  • Virology
  • Pathology

Background:

  • Human cytomegalovirus (HCMV) has been previously associated with atherosclerosis.
  • The precise role of local HCMV infection and its protein expression in vascular atherosclerosis remains incompletely understood.

Purpose of the Study:

  • To investigate the association between HCMV infection and the pathogenesis of atherosclerosis.
  • To detect HCMV nucleic acids and proteins in atherosclerotic peripheral artery specimens.

Main Methods:

  • Analysis of formalin-fixed, paraffin-embedded peripheral artery specimens from 15 atherosclerosis patients.
  • Pathological examination using H&E and Masson trichrome staining.
  • Detection of HCMV via in situ hybridization and immunohistochemistry for IE1 72 and pp65 proteins.

Main Results:

  • Histopathology revealed characteristic features of atherosclerosis in carotid arteries.
  • In situ hybridization detected no HCMV nucleic acid expression in any samples.
  • Immunohistochemistry showed negative IE1 72 protein expression but positive pp65 protein detection in 14 out of 15 cases.

Conclusions:

  • The high prevalence of pp65 protein in atherosclerotic arteries suggests a potential role for local HCMV infection in atherosclerosis.
  • Further research is necessary to elucidate the specific mechanisms linking HCMV to the development of atherosclerosis.

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