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Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Published on: August 20, 2017
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Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos
Jiangwei Lin1, Mona Khan1, Bolek Zapiec1
1Max Planck Research Unit for Neurogenetics, Max-von-Laue-Strasse 4, 60438 Frankfurt, Germany.
Scientific Reports
|December 20, 2016
Summary
Researchers successfully derived extraembryonic endoderm stem (XEN) cell lines from postimplantation mouse embryos. These novel post-XEN cells closely resemble those from preimplantation embryos and contribute to extraembryonic tissues in chimeras.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Mouse Embryology
Background:
- Stem cell lines like embryonic stem cells, epiblast stem cells, and trophoblast stem cells are derived from mouse embryos.
- The derivation of extraembryonic endoderm stem (XEN) cell lines from postimplantation embryos remained unestablished.
Purpose of the Study:
- To investigate the feasibility of deriving XEN cell lines from postimplantation mouse embryos.
- To characterize and compare XEN cell lines derived from postimplantation (post-XEN) and preimplantation (pre-XEN) embryos.
Main Methods:
- Derivation of XEN cell lines from 85 postimplantation embryos (E5.5-E6.5) and 69 preimplantation embryos (blastocyst stage).
- Immunofluorescence and NanoString gene expression analyses for cell characterization.
- In vivo assessment of post-XEN cell contribution to extraembryonic endoderm in blastocyst-injected chimeras.
Main Results:
- Successfully derived 77 XEN cell lines from postimplantation embryos, achieving a 100% derivation success rate.
- Post-XEN cell lines exhibited significant similarity to pre-XEN cell lines based on immunofluorescence and gene expression.
- Injected post-XEN cells contributed effectively to extraembryonic endoderm in E6.5 and E7.5 chimeras.
Conclusions:
- Extraembryonic endoderm stem cell lines can be reliably derived from postimplantation mouse embryos.
- Postimplantation-derived XEN cells are comparable to their preimplantation counterparts and retain developmental potential.
- This study expands the sources for XEN cell derivation, offering new avenues for developmental and stem cell research.
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