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Surface-Plasmon-Coupled Fluorescence Enhancement Based on Ordered Gold Nanorod Array Biochip for Ultrasensitive DNA
1Department of Biomedical Engineering, University of Texas at San Antonio , San Antonio, Texas 78249, United States.
Analytical Chemistry
|December 20, 2016
Summary
This study introduces a gold nanorod (GNR) array biochip that amplifies fluorescence signals for sensitive DNA detection. The novel GNR array enhances molecular beacon detection, overcoming limitations of existing methods.
Area of Science:
- Nanotechnology
- Biophysics
- Materials Science
Background:
- Localized surface plasmon resonance (LSPR) offers potential for signal amplification in biosensing.
- Molecular beacon assays require sensitive detection methods for accurate results.
- Existing plasmonic biosensors face challenges with signal enhancement and quenching effects.
Purpose of the Study:
- To develop and investigate a gold nanorod (GNR) array biochip for LSPR-coupled fluorescence enhancement.
- To amplify signals in molecular beacon detection for ultrasensitive DNA analysis.
- To explore the distance-dependent plasmon-induced fluorescence enhancement and its underlying mechanisms.
Main Methods:
- Fabrication of an ordered GNR assembly in a vertical standing array on a glass surface.
- Tuning the plasmonic response by selecting GNR size for spectral overlap with fluorophores (>600 nm).
- Systematic investigation of LSPR-coupled fluorescence enhancement and distance-dependent effects.
Main Results:
- The GNR array exhibited intensified LSPR compared to random nanorod ensembles.
- Plasmon-induced fluorescence enhancement was observed and found to be distance-dependent.
- The GNR array effectively overcame metal-induced quenching, even at close proximity.
- Enhancement correlated with spectral overlap, indicating surface-plasmon-enhanced excitation and radiative mechanisms.
Conclusions:
- The developed GNR array biochip provides a highly functional platform for molecular fluorescence enhancement.
- This technology enables ultrasensitive plasmonic DNA detection using molecular beacon assays.
- The ordered GNR array design significantly improves signal amplification in biosensing applications.
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