MgF₃^- and AlF₄^- transition state analogue complexes of yeast phosphoglycerate kinase

The phospho-transfer mechanism of yeast phosphoglycerate kinase (PGK) has been probed through formation of trifluoromagnesate (MgF₃^-) and tetrafluoroaluminate (AlF₄^-) transition state analogue complexes and analyzed using ¹⁹F, ¹H waterLOGSY and ¹H chemical shift perturbation NMR spectroscopy. We observed the first ¹⁹F NMR spectroscopic evidence for the formation of metal fluoride transition state analogues of yeast PGK and also observed significant changes to proton chemical shifts of PGK in the presence, but not in the absence, of fluoride upon titration of ligands, providing indirect evidence of the formation of a closed ternary transition state. WaterLOGSY NMR spectroscopy experiments using an uncompetitive model were used in an attempt to measure ligand binding affinities within the transition state analogue complexes.