Kinetics of desynchronization and distribution of generation times in synchronized cell populations

The kinetics of the distribution of generation times in synchronized cells can be analyzed by using Fourier transform analysis. Deviations of the experimental data from the curve of completely asynchronous growth reflect the degree of synchrony at a particular time. Fourier transform analysis of these deviations yields the average generation time as well as information on the distribution of generation times characterizing a synchronized culture. A detailed analysis of synchronized cell cultures does not provide any evidence for the existence of a subcycle or a polymodal distribution in generation times. The data do indicate that cell-cell interaction occurs at cell densities as low as 2.5 X 10(3)/cm2. It is also shown that the Eyring-Stover formalism for the dynamics of survival can correctly describe the distribution of the first round of cell divisions in a synchronized culture.