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Published on: July 3, 2020
cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans
Han Wang1,2, Jonathan Liu1,2, Shahla Gharib1,2
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Abstract:
The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.
