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Updated: Mar 9, 2026

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Published on: January 5, 2017
The importance of system band broadening in modern size exclusion chromatography
Alexandre Goyon1, Davy Guillarme1, Szabolcs Fekete1
1School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU - Rue Michel Servet, 1, 1211 Geneva 4, Switzerland.
Abstract:
In the last few years, highly efficient UHP-SEC columns packed with sub-3μm particles were commercialized by several providers. Besides the particle size reduction, the dimensions of modern SEC stationary phases (150×4.6mm) was also modified compared to regular SEC columns (300×6 or 300×8mm). Because the analytes are excluded from the pores in SEC, the retention factors are very low, ranging from -1
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